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> Brumer Harry
> Denman Stuart E. >
Enzymatic propertie...
Enzymatic properties of native and deglycosylated hybrid aspen (Populus tremula x tremuloides) xyloglucan endotransglycosylase 16A expressed in Pichia pastoris
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- Kallas, Åsa M. (author)
- KTH,Skolan för bioteknologi (BIO)
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- Piens, Kathleen (author)
- KTH,Skolan för bioteknologi (BIO)
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- Denman, Stuart E. (author)
- KTH,Skolan för bioteknologi (BIO)
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- Henriksson, Hongbin (author)
- KTH,Skolan för bioteknologi (BIO)
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- Fäldt, Jenny (author)
- KTH,Skolan för kemivetenskap (CHE)
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Johansson, Patrik (author)
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- Brumer, Harry (author)
- KTH,Skolan för bioteknologi (BIO)
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- Teeri, Tuula T. (author)
- KTH,Skolan för bioteknologi (BIO)
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(creator_code:org_t)
- 2005
- 2005
- English.
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In: Biochemical Journal. - 0264-6021. ; 390, s. 105-113
- Related links:
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http://www.biochemj....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The cDNA encoding a xyloglucan endotransglycosylase, PttXET16A, from hybrid aspen (Populus tremula x tremuloides) has been isolated from an expressed sequence tag library and expressed in the methylotrophic yeast Pichia pastoris. Sequence analysis indicated a high degree of similarity with other proteins in the XTH (xyloglucan transglycosylase/hydrolase) gene subfamily of GH16 (glycoside hydrolase family 16). In addition to the conserved GH16 catalytic sequence motif, PttXET16A contains a conserved N-glycosylation site situated proximal to the predicted catalytic residues. MS analysis indicated that the recombinant PttXET16A expressed in P. pastoris is heterogeneous due to the presence of variable N-glycosylation and incomplete cleavage of the a-factor secretion signal peptide. Removal of the N-glycan by endoglycosidase H treatment did not influence the catalytic activity significantly. Similarly, site-directed mutagenesis of Asn(93) to serine to remove the N-glycosylation site resulted in an enzyme which was comparable with the wild-type enzyme in specific activity and thermal stability but had clearly reduced solubility. Hydrolytic activity was detected neither in wild-type PttXET16A before or after enzymatic deglycosylation nor in PttXET16A N93S (Asn(93) -> Ser) mutant.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Keyword
- glycoside hydrolase family 16 (GH16)
- hybrid aspen (Populus tremula x tremuloides)
- Pichia pastoris
- protein glycosylation
- protein MS
- xyloglucan endotransglycosylase (XET)
- Bioengineering
- Bioteknik
Publication and Content Type
- ref (subject category)
- art (subject category)
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